Journal: Asian Pacific Journal of Tropical Biomedicine

Article Title: Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation

doi: 10.4103/apjtb.apjtb_518_24

Figure Lengend Snippet: Figure 6. Effects of L. draba extract on p53-positive cells in (A) kidney and liver tissues (DAB staining 400×, scale bar = 25 μm). (B) Quantitative results of immunohistochemical examination. Data are shown as mean ± SEM (n=6).

Article Snippet: The slides were then incubated in p53 rodent-specific monoclonal antibody (Cat. no. sc-98, Pab 1801, dilution 1꞉200, Santa Cruz Biotechnology) for 45 min at room temperature, then incubated for 5 min in 5% bovine serum albumin to block unbound antibodies, and finally in chromogen (3, 3’-diaminobenzidine) for 30 min at 37 曟. Counterstaining of the slides was performed with hematoxylin and 50 fields of view were randomly evaluated with a light microscope (Olympus CH3, Japan) attached to the camera (Moticam Technologies, Japan), and the p53 positive cells/total cells × 100 was calculated at 400× magnification and reported as mean (%) ± SEM[23].

Techniques: Staining, Immunohistochemical staining